Hotspot

psbA-trnK

LSC · 1419-1686 · 268 bp

Back to Arabidopsis

Pi 0.0148

MarkerSeek score 64.50

Primer available yes

Label psbA-trnK

Feature Metadata

label_name: psbA-trnK
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 26
pi: 0.0148
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK_p1	TACAGAAGCGACCCCATAGG	AAAATGCAAGCACGGTTTGG	55.0	45.0	58.6	58.4	431-451 bp	1.0	79.1
psbA-trnK_p2	ATCCAGTTACAGAAGCGACC	AAAATGCAAGCACGGTTTGG	50.0	45.0	57.3	58.4	438-458 bp	1.0	78.3
psbA-trnK_p3	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	457-477 bp	0.916667	74.8
psbA-trnK_p4	ACGGTTTTCAGTGCTGGTTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	389-411 bp	0.916667	73.8
psbA-trnK_p5	ACGGTTTTCAGTGCTGGTTA	TGAAAAATGCAAGCACGGTT	45.0	40.0	57.7	57.4	460-480 bp	0.916667	73.4