Hotspot

rpl16

LSC · 81447-82955 · 1509 bp

Back to Arabidella

Pi 0.0145

MarkerSeek score 61.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 56
pi: 0.0145
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	1969-2011 bp	1.0	84.4
rpl16_p2	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2228-2270 bp	1.0	83.8
rpl16_p3	ACACATCAATTCTCGAGCCC	GTATGGAGGGAATTGCACGT	50.0	50.0	58.0	58.0	2321-2363 bp	0.857143	78.2
rpl16_p4	GTCTCAATTCCCGTGGGATC	GTATGGAGGGAATTGCACGT	55.0	50.0	58.0	58.0	2580-2622 bp	0.857143	77.8
rpl16_p5	ATCGGCGATTACTAGCTCCT	GTATGGAGGGAATTGCACGT	50.0	50.0	58.1	58.0	2352-2394 bp	0.857143	77.7