Hotspot

rpl32-trnL

SSC · 114149-114932 · 784 bp

Back to Arabidella

Pi 0.0376

MarkerSeek score 76.10

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 73
pi: 0.0376
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	TCGTACGGATTTCAAACGGT	TGCAGACTGAGGCATTTTGT	45.0	45.0	57.6	58.0	1741 bp	0.142857	42.1
rpl32-trnL_p2	TCGTACGGATTTCAAACGGT	TAACCACTGGGACTGGAGAG	45.0	55.0	57.6	58.1	1774 bp	0.142857	41.7
rpl32-trnL_p3	TCGTACGGATTTCAAACGGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.6	57.9	1200 bp	0.142857	41.2
rpl32-trnL_p4	TGCAGAGACAGAAAACGTGA	TGCAGACTGAGGCATTTTGT	45.0	45.0	57.4	58.0	2084 bp	0.142857	40.8
rpl32-trnL_p5	TCGTACGGATTTCAAACGGT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.6	57.8	1193 bp	0.142857	40.7