Hotspot

ndhF-rpl32

SSC · 113155-113989 · 835 bp

Back to Arabidella

Pi 0.027

MarkerSeek score 67.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 835
variable_sites: 55
pi: 0.027
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	47.6	47.6	57.6	57.2	972-991 bp	1.0	59.8
ndhF-rpl32_p2	TTGTTGCTGTCGGAAAAAGT	ACGATGTCCAATATCCCTTCC	40.0	47.6	56.4	57.2	1020-1039 bp	1.0	58.3
ndhF-rpl32_p3	TGGAAGTGGAATGAAAGGGA	ACGATGTCCAATATCCCTTCC	45.0	47.6	56.3	57.2	971-990 bp	1.0	58.0
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGGA	CGATGTCCAATATCCCTTCCT	47.6	47.6	57.6	56.9	971-990 bp	1.0	57.6
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGGA	AACGATGTCCAATATCCCTTCC	47.6	45.5	57.6	57.9	973-992 bp	1.0	57.3