Hotspot

rps12-clpP

LSC · 69969-70149 · 181 bp

Back to Arabidella

Pi 0.0284

MarkerSeek score 63.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 15
pi: 0.0284
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	294-302 bp	1.0	81.6
rps12-clpP_p2	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	289-297 bp	1.0	81.1
rps12-clpP_p3	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	346-354 bp	1.0	81.0
rps12-clpP_p4	CCGAGTACATGTTCCTCGTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.8	57.9	347-355 bp	1.0	81.0
rps12-clpP_p5	CACCCGAGTACATGTTCCTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.7	57.9	350-358 bp	1.0	80.1