Hotspot

petA-psbJ

LSC · 62738-63724 · 987 bp

Back to Arabidella

Pi 0.0235

MarkerSeek score 63.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 987
variable_sites: 53
pi: 0.0235
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.0	56.8	1126-1165 bp	1.0	67.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.0	58.3	1127-1166 bp	1.0	67.3
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.2	56.8	1123-1162 bp	1.0	66.5
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.2	58.3	1124-1163 bp	1.0	66.2
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTG	50.0	47.6	58.0	56.5	1127-1166 bp	1.0	65.4