Hotspot

trnT-psbD

LSC · 31330-32461 · 1132 bp

Back to Arabidella

Pi 0.0163

MarkerSeek score 61.50

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 42
pi: 0.0163
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1230-1287 bp	1.0	85.8
trnT-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1233-1290 bp	1.0	85.6
trnT-psbD_p3	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1247-1304 bp	1.0	85.4
trnT-psbD_p4	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.8	58.0	1248-1305 bp	1.0	84.9
trnT-psbD_p5	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1207-1264 bp	1.0	84.1