Hotspot

trnE-trnT

LSC · 30436-31255 · 820 bp

Back to Arabidella

Pi 0.0166

MarkerSeek score 62.30

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 36
pi: 0.0166
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	889-906 bp	1.0	82.4
trnE-trnT_p2	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	868-885 bp	1.0	82.1
trnE-trnT_p3	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	865-882 bp	1.0	81.9
trnE-trnT_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	886-903 bp	1.0	81.3
trnE-trnT_p5	TGTCCTGAACCACTAGACGA	TTGAACCGATGACTTACGCC	50.0	50.0	57.7	58.3	891-908 bp	1.0	80.2