Hotspot

rpoB-trnC

LSC · 26145-27244 · 1100 bp

Back to Arabidella

Pi 0.0149

MarkerSeek score 63.40

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1100
variable_sites: 46
pi: 0.0149
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1188-1197 bp	1.0	82.1
rpoB-trnC_p2	CCCTCTTTTCCATCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1185-1194 bp	1.0	82.1
rpoB-trnC_p3	CTCTTTTCCATCCCCAAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1183-1192 bp	1.0	81.6
rpoB-trnC_p4	TCCCTCTTTTCCATCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1186-1195 bp	1.0	81.4
rpoB-trnC_p5	TTCCCTCTTTTCCATCCCCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1187-1196 bp	1.0	81.4