Hotspot

trnR-atpA

LSC · 9425-9715 · 291 bp

Back to Arabidella

Pi 0.0386

MarkerSeek score 64.30

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 27
pi: 0.0386
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GGTTCAAATCCTATTGGACGC	TCAAGAGCAACTGGAACGTT	47.6	45.0	57.3	57.7	323-359 bp	1.0	74.4
trnR-atpA_p2	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	413-416 bp	0.428571	54.2
trnR-atpA_p3	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	414-417 bp	0.428571	54.2
trnR-atpA_p4	GCGAAAAGCGTCCATTGTCT	TCAAGAGCAACTGGAACGTT	50.0	45.0	59.5	57.7	413-416 bp	0.428571	50.4
trnR-atpA_p5	GCGAAAAGCGTCCATTGTCT	TTCAAGAGCAACTGGAACGT	50.0	45.0	59.5	57.7	414-417 bp	0.428571	50.4