Hotspot

trnS-trnT

LSC · 7774-8432 · 659 bp

Back to Arabidella

Pi 0.0267

MarkerSeek score 70.90

Primer available yes

Label trnS-trnT

Feature Metadata

label_name: trnS-trnT
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 40
pi: 0.0267
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnT_p1	CCAACGCTTTAGTCCACTCA	GGAACGAATCACACTTTTACCA	50.0	40.9	57.8	56.9	720-748 bp	1.0	60.4
trnS-trnT_p2	AACGCTTTAGTCCACTCAGC	GGAACGAATCACACTTTTACCA	50.0	40.9	58.2	56.9	718-746 bp	1.0	60.1
trnS-trnT_p3	CCAACGCTTTAGTCCACTCA	AGGGATTAAAGGAACGAATCACA	50.0	39.1	57.8	57.8	730-758 bp	1.0	59.6
trnS-trnT_p4	AACGCTTTAGTCCACTCAGC	AGGGATTAAAGGAACGAATCACA	50.0	39.1	58.2	57.8	728-756 bp	1.0	59.3
trnS-trnT_p5	ACACACCGAGAAAGGGAAAG	GGAACGAATCACACTTTTACCA	50.0	40.9	57.7	56.9	797 bp	0.142857	19.0