Hotspot

rps16-trnQ

LSC · 6098-6518 · 421 bp

Back to Arabidella

Pi 0.0249

MarkerSeek score 62.30

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 421
variable_sites: 26
pi: 0.0249
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	488-494 bp	1.0	85.3
rps16-trnQ_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	498-504 bp	1.0	84.4
rps16-trnQ_p3	TCAATTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	495-501 bp	1.0	81.0
rps16-trnQ_p4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	492-498 bp	1.0	80.5
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	518-524 bp	1.0	80.1