Hotspot

rpl32-trnL-UAG

SSC · 120385-120811 · 427 bp

Back to Aquilegia

Pi 0.00937
MarkerSeek score 58.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
427
variable_sites
32
pi
0.00937
unique_haplotype_count
53
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGTATGCGTAAAAATTTTTGGA AGCGTGTCTACCGATTTCAC 27.3 50.0 52.9 58.0 437-518 bp 0.971429 48.0
rpl32-trnL-UAG_p2 AGTATGCGTAAAAATTTTTGGA CAGCGTGTCTACCGATTTCA 27.3 50.0 52.9 58.0 438-519 bp 0.971429 48.0
rpl32-trnL-UAG_p3 AGTATGCGTAAAAATTTTTGGAA AGCGTGTCTACCGATTTCAC 26.1 50.0 53.8 58.0 437-518 bp 0.971429 48.0
rpl32-trnL-UAG_p4 AGTATGCGTAAAAATTTTTGGA TCCTAAGAGCAGCGTGTCTA 27.3 50.0 52.9 57.9 447-528 bp 0.971429 47.9
rpl32-trnL-UAG_p5 AGTATGCGTAAAAATTTTTGGA TTATAAGATGCCGCCACTCG 27.3 50.0 52.9 57.9 496-577 bp 0.971429 47.5