Hotspot

rpl2-rpl23

IRb · 90485-90531 · 47 bp

Back to Aquilegia

Pi 0.0145

MarkerSeek score 60.90

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 47
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	GCTCGGGGTAGAAGTTTTGT	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	133-201 bp	1.0	86.7
rpl2-rpl23_p2	TGCTCGGGGTAGAAGTTTTG	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	134-202 bp	1.0	86.6
rpl2-rpl23_p3	GTGCTCGGGGTAGAAGTTTT	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	135-203 bp	1.0	86.6
rpl2-rpl23_p4	TGCTCGGGGTAGAAGTTTTG	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	141-209 bp	1.0	85.8
rpl2-rpl23_p5	TTACCACAATGACGCTGTCC	CGCTTCAACCGGGTTATTCT	50.0	50.0	58.2	58.3	213-281 bp	1.0	85.5