Hotspot

trnN-GUU-infA

LSC · 84833-85351 · 519 bp

Back to Aquilegia

Pi 0.0112

MarkerSeek score 63.10

Primer available yes

Label trnN-GUU-infA

Feature Metadata

label_name: trnN-GUU-infA
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 35
pi: 0.0112
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-infA_p1	CCGTTGTTCCTCAGTAGCTC	GGGAGGATCTGCCGAAATTT	55.0	50.0	58.0	57.9	474-709 bp	1.0	88.5
trnN-GUU-infA_p2	CGAATTTGCCGTTGTTCCTC	GGGAGGATCTGCCGAAATTT	50.0	50.0	58.0	57.9	482-717 bp	1.0	88.4
trnN-GUU-infA_p3	ATTTGCCGTTGTTCCTCAGT	GGGAGGATCTGCCGAAATTT	45.0	50.0	58.0	57.9	479-714 bp	1.0	88.3
trnN-GUU-infA_p4	CCGTTGTTCCTCAGTAGCTC	TAGGTTATGGTTCGGGGAGG	55.0	55.0	58.0	58.2	488-723 bp	1.0	87.3
trnN-GUU-infA_p5	TGGGTTGAGAACGGGAATTG	GGGAGGATCTGCCGAAATTT	50.0	50.0	58.1	57.9	515-750 bp	0.971429	86.5