Hotspot

rpl36-trnN-GUU

LSC · 84106-84760 · 655 bp

Back to Aquilegia

Pi 0.0043
MarkerSeek score 51.70
Primer available yes
Label rpl36-trnN-GUU

Feature Metadata

label_name
rpl36-trnN-GUU
feature_type
igs
region
LSC
length_bp
655
variable_sites
26
pi
0.0043
unique_haplotype_count
41
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-trnN-GUU_p1 GTCTCTGTTTATGCCTCGGG ATCTCCCCAGGTAGGATTCG 55.0 55.0 58.1 58.0 823-840 bp 1.0 84.7
rpl36-trnN-GUU_p2 TCTGTTTATGCCTCGGGTTG ATCTCCCCAGGTAGGATTCG 50.0 55.0 57.9 58.0 820-837 bp 1.0 84.3
rpl36-trnN-GUU_p3 CCTCGGGTTGGAGCAAATTA ATCTCCCCAGGTAGGATTCG 50.0 55.0 57.9 58.0 810-827 bp 1.0 84.3
rpl36-trnN-GUU_p4 TTTTACGAATGGAAGCCCGT ATCTCCCCAGGTAGGATTCG 45.0 55.0 57.8 58.0 745-762 bp 1.0 84.2
rpl36-trnN-GUU_p5 ACGGATCAGTCGGCATTTTT ATCTCCCCAGGTAGGATTCG 45.0 55.0 58.2 58.0 771-788 bp 1.0 84.1