Hotspot

accD-trnG-GCC

LSC · 73793-75133 · 1341 bp

Back to Aquilegia

Pi 0.00211

MarkerSeek score 58.40

Primer available yes

Label accD-trnG-GCC

Feature Metadata

label_name: accD-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1341
variable_sites: 29
pi: 0.00211
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-trnG-GCC_p1	CAACATCCAATTGAACCGCC	CTTCACGATAGGCGGATAGC	50.0	55.0	58.0	58.0	1251-1482 bp	1.0	85.4
accD-trnG-GCC_p2	CAATTGAACCGCCGTCTTTC	CTTCACGATAGGCGGATAGC	50.0	55.0	58.0	58.0	1244-1475 bp	1.0	85.2
accD-trnG-GCC_p3	AGCGATTTACTTAGCCCACG	CTTCACGATAGGCGGATAGC	50.0	55.0	58.1	58.0	1295-1526 bp	1.0	84.9
accD-trnG-GCC_p4	ATTCCAATGGGGCCAATACC	CTTCACGATAGGCGGATAGC	50.0	55.0	57.9	58.0	1319-1550 bp	1.0	84.6
accD-trnG-GCC_p5	CAACATCCAATTGAACCGCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.0	57.8	1217-1448 bp	1.0	84.2