Hotspot

clpP-rps12

LSC · 60511-61729 · 1219 bp

Back to Aquilegia

Pi 0.00635

MarkerSeek score 60.20

Primer available yes

Label clpP-rps12

Feature Metadata

label_name: clpP-rps12
feature_type: igs
region: LSC
length_bp: 1219
variable_sites: 43
pi: 0.00635
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rps12_p1	ATGCTGTAGCGGACGAAAAT	GAGCGGGGGATTTTGTTACA	45.0	50.0	58.0	58.2	1324-2350 bp	1.0	84.4
clpP-rps12_p2	ATGCTGTAGCGGACGAAAAT	AGAGCGGGGGATTTTGTTAC	45.0	50.0	58.0	57.9	1325-2351 bp	0.985714	84.1
clpP-rps12_p3	GACTTGGACCTCCCATCCTA	AGAGCGGGGGATTTTGTTAC	55.0	50.0	57.8	57.9	1296-2322 bp	0.985714	83.0
clpP-rps12_p4	CTTGGACTTGGACTTGGACC	AGAGCGGGGGATTTTGTTAC	55.0	50.0	58.1	57.9	1373-2321 bp	0.7	71.1
clpP-rps12_p5	CTTGGACTTGGACTTGGACC	GAGCGGGGGATTTTGTTACA	55.0	50.0	58.1	58.2	1372-2320 bp	0.7	70.8