Hotspot

psbM-trnD-GUC

LSC · 30927-32024 · 1098 bp

Back to Aquilegia

Pi 0.00386

MarkerSeek score 54.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1098
variable_sites: 33
pi: 0.00386
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1190-1219 bp	1.0	63.1
psbM-trnD-GUC_p2	TTGACTGACCGTTTTTACGT	TTCAATTGGTCAGAGCACCG	40.0	50.0	55.6	58.5	1239-1268 bp	1.0	61.3
psbM-trnD-GUC_p3	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1197-1226 bp	1.0	59.9
psbM-trnD-GUC_p4	TTTGACTGACCGTTTTTACGT	TTCAATTGGTCAGAGCACCG	38.1	50.0	56.3	58.5	1240-1269 bp	1.0	59.5
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1191-1220 bp	1.0	58.9