Hotspot

petN-psbM

LSC · 29678-30821 · 1144 bp

Back to Aquilegia

Pi 0.00225

MarkerSeek score 52.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 23
pi: 0.00225
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1287-1315 bp	1.0	71.7
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1293-1321 bp	1.0	70.2
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1233-1261 bp	1.0	69.0
petN-psbM_p4	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1316-1344 bp	1.0	68.7
petN-psbM_p5	AGGGGGCATAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1317-1345 bp	1.0	68.6