Hotspot

trnH-GUG-rpl16

LSC · 346-565 · 220 bp

Back to Aquilegia

Pi 0.013

MarkerSeek score 50.80

Primer available yes

Label trnH-GUG-rpl16

Feature Metadata

label_name: trnH-GUG-rpl16
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 20
pi: 0.013
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-rpl16_p1	TGAAATAGGATGGGCGAACG	CGAAATCGGATGCTTTTGGG	50.0	50.0	58.1	57.8	320-352 bp	0.985714	86.7
trnH-GUG-rpl16_p2	CAATCCACTGCCTTCATCCA	CGAAATCGGATGCTTTTGGG	50.0	50.0	57.9	57.8	269-301 bp	0.985714	86.7
trnH-GUG-rpl16_p3	AATCCACTGCCTTCATCCAC	CGAAATCGGATGCTTTTGGG	50.0	50.0	57.9	57.8	268-300 bp	0.985714	86.7
trnH-GUG-rpl16_p4	ACAATCCACTGCCTTCATCC	CGAAATCGGATGCTTTTGGG	50.0	50.0	57.9	57.8	270-302 bp	0.985714	86.7
trnH-GUG-rpl16_p5	AAATAGGATGGGCGAACGAC	CGAAATCGGATGCTTTTGGG	50.0	50.0	58.1	57.8	318-350 bp	0.985714	86.5