Hotspot

rps16

LSC · 5088-6228 · 1141 bp

Back to Aquilaria

Pi 0.00361

MarkerSeek score 52.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1141
variable_sites: 17
pi: 0.00361
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTCATCCACACCTCTTT	CTACGCACAGACTGAACCAA	45.0	50.0	56.1	57.9	1293-1299 bp	1.0	65.0
rps16_p2	TGAGTCATCCACACCTCTTTT	CTACGCACAGACTGAACCAA	42.9	50.0	56.9	57.9	1293-1299 bp	1.0	63.3
rps16_p3	ATGAGTCATCCACACCTCTT	CTACGCACAGACTGAACCAA	45.0	50.0	55.8	57.9	1294-1300 bp	1.0	63.1
rps16_p4	TGAGTCATCCACACCTCTTT	CGCACAGACTGAACCAATGA	45.0	50.0	56.1	58.5	1290-1296 bp	1.0	62.3
rps16_p5	ATGAGTCATCCACACCTCTTT	CTACGCACAGACTGAACCAA	42.9	50.0	56.6	57.9	1294-1300 bp	1.0	61.5