Hotspot

rpl2-rpl23

IRb · 88872-88889 · 18 bp

Back to Aquilaria

Pi 0.0505

MarkerSeek score 57.00

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: IRb
length_bp: 18
variable_sites: 3
pi: 0.0505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	CACTTGACTGTCTACGGCTC	GAATGGTTAAAGCGCCCAAC	55.0	50.0	58.0	58.0	598 bp	1.0	80.5
rpl2-rpl23_p2	CCACAACGATGCTGTCCATA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	645 bp	1.0	80.4
rpl2-rpl23_p3	TTCTGTCGGTGCTTGAAACA	GAATGGTTAAAGCGCCCAAC	45.0	50.0	58.0	58.0	1154 bp	1.0	80.0
rpl2-rpl23_p4	CACTTGACTGTCTACGGCTC	CCTGTTAAGCATCCATGGCT	55.0	50.0	58.0	57.9	618 bp	1.0	80.0
rpl2-rpl23_p5	TGTGAATAACCCGTCCTCCT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.1	58.0	1200 bp	1.0	79.8