Hotspot

ndhC-trnV-UAC

LSC · 53720-54224 · 505 bp

Back to Aquilaria

Pi 0.0215

MarkerSeek score 64.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 30
pi: 0.0215
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATGGGGCCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	648-649 bp	1.0	82.0
ndhC-trnV-UAC_p2	ATGGGGCCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	638-639 bp	1.0	81.9
ndhC-trnV-UAC_p3	AATGGGGCCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	639-640 bp	1.0	80.2
ndhC-trnV-UAC_p4	TGGGGCCTAAAACTCCAGAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	637-638 bp	1.0	80.1
ndhC-trnV-UAC_p5	GGGGCCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	636-637 bp	1.0	79.7