Hotspot

trnF-GAA-ndhJ

LSC · 51465-51981 · 517 bp

Back to Aquilaria

Pi 0.00591

MarkerSeek score 44.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 517
variable_sites: 12
pi: 0.00591
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CACGGCTGAAGCGTATCTTA	55.0	50.0	58.0	57.8	682-690 bp	1.0	80.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CACGGCTGAAGCGTATCTTA	50.0	50.0	58.1	57.8	646-654 bp	1.0	79.7
trnF-GAA-ndhJ_p3	TGGTAATGGTCGGGATAGCT	CACGGCTGAAGCGTATCTTA	50.0	50.0	57.9	57.8	693-701 bp	1.0	79.2
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAGAATCATCCACGGCTGAA	55.0	50.0	58.0	57.7	692-700 bp	1.0	79.1
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	AGAATCATCCACGGCTGAAG	55.0	50.0	58.0	57.7	691-699 bp	1.0	79.0