Hotspot

psaA-ycf3

LSC · 44290-45262 · 973 bp

Back to Aquilaria

Pi 0.00972

MarkerSeek score 61.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 25
pi: 0.00972
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	1132-1140 bp	1.0	81.3
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GATTACGAGGCGGTTCGAAT	50.0	50.0	57.9	58.2	1074-1082 bp	1.0	80.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATAGCGCTTACTCCTGGGAA	50.0	50.0	57.9	58.2	1121-1129 bp	1.0	80.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCTGGGAAT	50.0	50.0	57.9	58.2	1120-1128 bp	1.0	80.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	50.0	50.0	57.9	57.7	1126-1134 bp	1.0	79.3