Hotspot

petN-psbM

LSC · 30347-31451 · 1105 bp

Back to Aquilaria

Pi 0.00474

MarkerSeek score 56.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 21
pi: 0.00474
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1267-1271 bp	1.0	78.4
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1268-1272 bp	1.0	77.4
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	1206-1210 bp	1.0	76.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	1266-1270 bp	1.0	76.6
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.6	57.6	1207-1211 bp	1.0	75.9