Hotspot

rpoC1-rpoB

LSC · 25086-25114 · 29 bp

Back to Aquilaria

Pi 0.0213

MarkerSeek score 53.70

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 29
variable_sites: 2
pi: 0.0213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGGTTTTGTCACCTCTCCAA	TTGGCTTTGGAACTGAACCA	50.0	45.0	57.6	57.8	218-222 bp	1.0	76.7
rpoC1-rpoB_p2	ATTTGTTGAGGCGAAACCGA	TTGGCTTTGGAACTGAACCA	45.0	45.0	58.4	57.8	157-161 bp	1.0	76.6
rpoC1-rpoB_p3	TTTGTTGAGGCGAAACCGAT	TTGGCTTTGGAACTGAACCA	45.0	45.0	58.4	57.8	156-160 bp	1.0	76.6
rpoC1-rpoB_p4	GAGGCGAAACCGATCCTATT	TTGGCTTTGGAACTGAACCA	50.0	45.0	57.5	57.8	150-154 bp	1.0	75.6
rpoC1-rpoB_p5	GGGTTTTGTCACCTCTCCAA	TGGCTTTGGAACTGAACCAT	50.0	45.0	57.6	57.6	217-221 bp	1.0	74.8