Hotspot

rps16-trnQ-UUG

LSC · 6229-7412 · 1184 bp

Back to Aquilaria

Pi 0.00424

MarkerSeek score 55.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 19
pi: 0.00424
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	TTTGGTCCCGCTATTCGAAG	45.0	50.0	58.1	58.0	1272-1446 bp	1.0	80.1
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	TTTGGTCCCGCTATTCGAAG	50.0	50.0	58.2	58.0	1283-1457 bp	1.0	79.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTTTTGGTCCCGCTATTCGA	45.0	50.0	58.1	58.3	1274-1448 bp	1.0	78.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1313-1487 bp	0.909091	76.5
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1324-1498 bp	0.909091	76.0