Hotspot

trnK-UUU-rps16

LSC · 4383-5087 · 705 bp

Back to Aquilaria

Pi 0.00943

MarkerSeek score 54.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 21
pi: 0.00943
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAGCCGAGTACTCTACCGTT	TATTTCCTTGAAAGGGGCGC	50.0	50.0	58.2	58.5	806-816 bp	1.0	77.6
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GCGGGGTTTTACGGAACTTA	50.0	50.0	58.2	58.2	755-760 bp	0.909091	75.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GCGGGGTTTTACGGAACTTA	50.0	50.0	58.2	58.2	754-759 bp	0.909091	75.7
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GCGGGGTTTTACGGAACTTA	50.0	50.0	58.3	58.2	764-769 bp	0.909091	74.8
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	GCGGGGTTTTACGGAACTTA	50.0	50.0	57.5	58.2	741-746 bp	0.909091	73.4