Hotspot

ycf1

IRb · 112547-113688 · 1142 bp

Back to Apostasia

Pi 0.0414

MarkerSeek score 68.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1142
variable_sites: 47
pi: 0.0414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	ACTCGACACCATTGTGCTTT	55.0	45.0	58.0	58.0	2249 bp	0.5	59.7
ycf1_p2	GCTTCCTTCGTAACTCCAGG	ACTCGACACCATTGTGCTTT	55.0	45.0	58.0	58.0	2169 bp	0.5	59.6
ycf1_p3	AAAGCGAGCCCTTTCATTCA	ACTCGACACCATTGTGCTTT	45.0	45.0	58.1	58.0	2361 bp	0.5	59.0
ycf1_p4	AAGCGAGCCCTTTCATTCAA	ACTCGACACCATTGTGCTTT	45.0	45.0	58.1	58.0	2360 bp	0.5	59.0
ycf1_p5	TCAAAGCGAGCCCTTTCATT	ACTCGACACCATTGTGCTTT	45.0	45.0	58.1	58.0	2363 bp	0.5	59.0