Hotspot

rps16

LSC · 5781-7069 · 1289 bp

Back to Apostasia

Pi 0.0305

MarkerSeek score 65.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1289
variable_sites: 38
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGAATTGCGCTGTACAACT	CAAGTGGTAAGGCAATGGGT	45.0	50.0	58.1	58.1	2666-2808 bp	1.0	84.0
rps16_p2	GGAATTGCGCTGTACAACTG	CAAGTGGTAAGGCAATGGGT	50.0	50.0	57.8	58.1	2665-2807 bp	1.0	82.8
rps16_p3	GAATTGCGCTGTACAACTGC	CAAGTGGTAAGGCAATGGGT	50.0	50.0	58.4	58.1	2664-2806 bp	1.0	81.7
rps16_p4	TGGAATTGCGCTGTACAACT	GGGGCCCCATTTTTACTCTT	45.0	50.0	58.1	57.8	2211 bp	0.5	57.1
rps16_p5	GGAATTGCGCTGTACAACTG	GGGGCCCCATTTTTACTCTT	50.0	50.0	57.8	57.8	2210 bp	0.5	56.0