Hotspot

petD

LSC · 78356-79903 · 1548 bp

Back to Apostasia

Pi 0.0233

MarkerSeek score 66.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1548
variable_sites: 33
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGACATGTTGTTTGGATACTTCTC	TGATGGTTGAATCAATACTTCTGT	37.5	33.3	56.9	56.4	1664 bp	0.5	20.0
petD_p2	GACATGTTGTTTGGATACTTCTCT	TGATGGTTGAATCAATACTTCTGT	37.5	33.3	56.9	56.4	1663 bp	0.5	20.0
petD_p3	ACATGTTGTTTGGATACTTCTCT	TGATGGTTGAATCAATACTTCTGT	34.8	33.3	55.9	56.4	1662 bp	0.5	20.0
petD_p4	AGACATGTTGTTTGGATACTTCT	TGATGGTTGAATCAATACTTCTGT	34.8	33.3	55.9	56.4	1664 bp	0.5	20.0
petD_p5	AGACATGTTGTTTGGATACTTCTCT	TGATGGTTGAATCAATACTTCTGT	36.0	33.3	58.2	56.4	1664 bp	0.5	20.0