Hotspot

psbT-psbN

LSC · 76049-76114 · 66 bp

Back to Apostasia

Pi 0.106

MarkerSeek score 84.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 7
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CCGCCGAAAGTTCCAACTAA	GGGTACGCCTTATATACCGC	50.0	55.0	58.5	57.7	174 bp	1.0	77.1
psbT-psbN_p2	CCGCCGAAAGTTCCAACTAA	TCGCCATCTCTATATCGGGT	50.0	50.0	58.5	57.5	212 bp	1.0	74.7
psbT-psbN_p3	TTTTTCGAGAACCGCCGAA	GGGTACGCCTTATATACCGC	47.4	55.0	58.0	57.7	185 bp	1.0	72.7
psbT-psbN_p4	CCGCCGAAAGTTCCAACTAA	GTCGCCATCTCTATATCGGG	50.0	55.0	58.5	57.1	213 bp	1.0	71.9
psbT-psbN_p5	CCGCCGAAAGTTCCAACTAA	GGGGTACGCCTTATATACCG	50.0	55.0	58.5	57.1	175 bp	1.0	71.7