Hotspot

psbB-psbT

LSC · 74656-75940 · 1285 bp

Back to Apostasia

Pi 0.0656

MarkerSeek score 84.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 71
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTGCCAGAACCTTGTTCAGA	TTAGTTGGAACTTTCGGCGG	50.0	50.0	58.0	58.5	1467-1499 bp	1.0	83.7
psbB-psbT_p2	TGCCAGAACCTTGTTCAGAG	TTAGTTGGAACTTTCGGCGG	50.0	50.0	57.7	58.5	1466-1498 bp	1.0	81.9
psbB-psbT_p3	TCAGAGATGTTTTTGCCGGT	TTAGTTGGAACTTTCGGCGG	45.0	50.0	57.7	58.5	1452-1484 bp	1.0	81.8
psbB-psbT_p4	GGGACCCAACTACAAGGAGA	TTAGTTGGAACTTTCGGCGG	55.0	50.0	58.3	58.5	1380-1412 bp	1.0	81.4
psbB-psbT_p5	GCCAGAACCTTGTTCAGAGA	TTAGTTGGAACTTTCGGCGG	50.0	50.0	57.4	58.5	1465-1497 bp	1.0	79.5