Hotspot

rps4-trnT-UGU

LSC · 46207-46590 · 384 bp

Back to Apostasia

Pi 0.0507

MarkerSeek score 78.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 18
pi: 0.0507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCAGACGGCGTATCTTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	468-494 bp	1.0	83.8
rps4-trnT-UGU_p2	CCCAGACGGCGTATCTTTTT	GGTTCGATTCCGATAGCTGG	50.0	55.0	58.3	58.2	427-453 bp	1.0	83.4
rps4-trnT-UGU_p3	GTTAATCCCGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	486-512 bp	1.0	83.3
rps4-trnT-UGU_p4	CCCAGACGGCGTATCTTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	436-462 bp	1.0	83.2
rps4-trnT-UGU_p5	GTTAATCCCGGTAAAGCCCC	GGTTCGATTCCGATAGCTGG	55.0	55.0	58.3	58.2	445-471 bp	1.0	82.9