Hotspot

atpI-atpH

LSC · 39506-40247 · 742 bp

Back to Apostasia

Pi 0.0279

MarkerSeek score 77.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 20
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	AACTTTGGCTGCGGCTTATA	AGCCAATCCAGCAGCAATAA	45.0	45.0	57.9	57.9	823-839 bp	1.0	82.2
atpI-atpH_p2	AACTTTGGCTGCGGCTTATA	CAGCAGCAATAACAGAAGCG	45.0	50.0	57.9	57.8	815-831 bp	1.0	82.0
atpI-atpH_p3	AACTTTGGCTGCGGCTTATA	GCAGCAGTACCTTGACCAAT	45.0	50.0	57.9	58.2	870-886 bp	1.0	81.7
atpI-atpH_p4	AACTTTGGCTGCGGCTTATA	GCCAATCCAGCAGCAATAAC	45.0	50.0	57.9	57.8	822-838 bp	1.0	81.6
atpI-atpH_p5	AACTTTGGCTGCGGCTTATA	GCGGCAGAAATAAGTGGACT	45.0	50.0	57.9	58.3	798-814 bp	1.0	81.4