Hotspot

rpoC1-rpoC2

LSC · 33110-33350 · 241 bp

Back to Apostasia

Pi 0.0948

MarkerSeek score 74.10

Primer available yes

Label rpoC1-rpoC2

Feature Metadata

label_name: rpoC1-rpoC2
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 20
pi: 0.0948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoC2_p1	TGATGGGGATCAAATGGCTG	CCGCGTGTACATTCCCATAA	50.0	50.0	57.9	58.1	1084-1086 bp	1.0	83.4
rpoC1-rpoC2_p2	GATGGGGATCAAATGGCTGT	CCGCGTGTACATTCCCATAA	50.0	50.0	57.9	58.1	1083-1085 bp	1.0	83.3
rpoC1-rpoC2_p3	TGATGGGGATCAAATGGCTG	TCTAGCTCCCGAAAAGGACA	50.0	50.0	57.9	58.1	1215-1217 bp	1.0	82.8
rpoC1-rpoC2_p4	TGATGGGGATCAAATGGCTG	GTCTACGGGTGAGGTATCCA	50.0	55.0	57.9	57.9	1420-1422 bp	1.0	82.3
rpoC1-rpoC2_p5	GATGGGGATCAAATGGCTGT	GTCTACGGGTGAGGTATCCA	50.0	55.0	57.9	57.9	1419-1421 bp	1.0	82.3