Hotspot

trnG-UCC-psbZ

LSC · 17410-17711 · 302 bp

Back to Apostasia

Pi 0.0321

MarkerSeek score 72.80

Primer available yes

Label trnG-UCC-psbZ

Feature Metadata

label_name: trnG-UCC-psbZ
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 9
pi: 0.0321
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbZ_p1	AATCGAACCCGCATCTTCTC	CTTCCGATGGTTGGTCAAGT	50.0	50.0	58.1	57.8	447-464 bp	1.0	83.5
trnG-UCC-psbZ_p2	GAATCGAACCCGCATCTTCT	CTTCCGATGGTTGGTCAAGT	50.0	50.0	58.1	57.8	448-465 bp	1.0	83.5
trnG-UCC-psbZ_p3	ACATTACTTCACCATGGGGC	CTTCCGATGGTTGGTCAAGT	50.0	50.0	57.9	57.8	477-501 bp	1.0	82.8
trnG-UCC-psbZ_p4	AATCGAACCCGCATCTTCTC	TTGCTTCTTCCGATGGTTGG	50.0	50.0	58.1	58.5	453-470 bp	1.0	81.2
trnG-UCC-psbZ_p5	GAATCGAACCCGCATCTTCT	TTGCTTCTTCCGATGGTTGG	50.0	50.0	58.1	58.5	454-471 bp	1.0	81.2