Hotspot

psbK-psbI

LSC · 8236-8408 · 173 bp

Back to Apostasia

Pi 0.0299

MarkerSeek score 72.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 5
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTATTCCTCACGCCCAGG	50.0	50.0	57.9	57.9	316-637 bp	1.0	80.4
psbK-psbI_p2	ATGCCCCTTTCAATCCCATC	TTTTATTCCTCACGCCCAGG	50.0	50.0	57.9	57.9	384-705 bp	1.0	79.9
psbK-psbI_p3	TTCACCAAATTACCCGAGGC	TTTTATTCCTCACGCCCAGG	50.0	50.0	58.2	57.9	406-727 bp	1.0	79.2
psbK-psbI_p4	CCCATCGTAGACGTTATGCC	TTTTATTCCTCACGCCCAGG	55.0	50.0	58.2	57.9	370-691 bp	1.0	78.9
psbK-psbI_p5	CACCAAATTACCCGAGGCAT	TTTTATTCCTCACGCCCAGG	50.0	50.0	58.2	57.9	404-725 bp	1.0	78.7