Hotspot

rps16-trnQ-UUG

LSC · 7070-7695 · 626 bp

Back to Apostasia

Pi 0.0364

MarkerSeek score 72.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 22
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	CAAGTGGTAAGGCAATGGGT	45.0	50.0	58.1	58.1	752-774 bp	1.0	84.2
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.1	57.3	718-740 bp	1.0	79.8
rps16-trnQ-UUG_p3	TATCCTTCAAGTCGCACGTT	CAAGTGGTAAGGCAATGGGT	45.0	50.0	57.3	58.1	760-782 bp	1.0	79.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	AAGTGGTAAGGCAATGGGTT	45.0	45.0	58.1	57.3	751-773 bp	1.0	79.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AGTGGTAAGGCAATGGGTTT	45.0	45.0	58.1	57.3	750-772 bp	1.0	79.3