Hotspot

matK-rps16

LSC · 3532-5780 · 2249 bp

Back to Apostasia

Pi 0.0371

MarkerSeek score 65.00

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 2249
variable_sites: 71
pi: 0.0371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TCGCGACGAGAATGGAATTT	TCATTTTTGCTCGAGCCGTA	45.0	45.0	58.0	57.9	2695-2870 bp	1.0	85.7
matK-rps16_p2	TCGCGACGAGAATGGAATTT	TTTTTGCTCGAGCCGTATGA	45.0	45.0	58.0	57.9	2692-2867 bp	1.0	85.7
matK-rps16_p3	TCGCGACGAGAATGGAATTT	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.0	57.9	2587-2763 bp	1.0	85.4
matK-rps16_p4	TCGCGACGAGAATGGAATTT	GGTGCTCAACCTACAGGAAC	45.0	55.0	58.0	58.2	2481-2657 bp	1.0	85.1
matK-rps16_p5	ACTAATCGCGACGAGAATGG	TTTTTGCTCGAGCCGTATGA	50.0	45.0	57.9	57.9	2697-2872 bp	1.0	84.8