Hotspot

ndhF

SSC · 110498-112714 · 2217 bp

Back to Aponogeton

Pi 0.0141

MarkerSeek score 52.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 47
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTTCCTAATGGCTCCGTTCC	TCTTGACCGACTGATTTGGT	55.0	45.0	58.0	56.8	2965-2992 bp	1.0	73.8
ndhF_p2	CTCCGTTCCATGCCTCATTT	TCTTGACCGACTGATTTGGT	50.0	45.0	58.2	56.8	2954-2981 bp	1.0	72.2
ndhF_p3	TCCGTTCCATGCCTCATTTC	TCTTGACCGACTGATTTGGT	50.0	45.0	58.2	56.8	2953-2980 bp	1.0	72.1
ndhF_p4	CGATCGCAAAATGACTAGGC	TGAGTTGGACTTTTGGCAGA	50.0	45.0	57.3	57.3	2667 bp	0.333333	42.3
ndhF_p5	CGCAAAATGACTAGGCATCC	TGAGTTGGACTTTTGGCAGA	50.0	45.0	57.2	57.3	2663 bp	0.333333	41.4