Hotspot

rrn5-rrn4.5

IRa · 131616-131842 · 227 bp

Back to Aponogeton

Pi 0.0499

MarkerSeek score 71.80

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 227
variable_sites: 17
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	344 bp	1.0	83.3
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	342 bp	1.0	83.3
rrn5-rrn4.5_p3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	340 bp	1.0	83.3
rrn5-rrn4.5_p4	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	368 bp	1.0	83.1
rrn5-rrn4.5_p5	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	370 bp	1.0	83.0