Hotspot

trnN-GUU-ndhF

IRb · 109869-110497 · 629 bp

Back to Aponogeton

Pi 0.036

MarkerSeek score 72.30

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 629
variable_sites: 31
pi: 0.036
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TCCTAACTCCTAACGGCCAA	TCCGCTACTTGTATTGGCAC	50.0	50.0	58.1	58.0	1337-1370 bp	1.0	85.1
trnN-GUU-ndhF_p2	CTCCTAACGGCCAAAGCTAG	TCCGCTACTTGTATTGGCAC	55.0	50.0	58.1	58.0	1331-1364 bp	1.0	85.1
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	TCCGCTACTTGTATTGGCAC	50.0	50.0	58.0	58.0	1643-1676 bp	1.0	84.2
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	CCGCTACTTGTATTGGCACT	50.0	50.0	58.0	58.0	1642-1675 bp	1.0	84.2
trnN-GUU-ndhF_p5	TGGAACTTGGGTATGGGTCT	TCCGCTACTTGTATTGGCAC	50.0	50.0	57.9	58.0	1780-1813 bp	1.0	83.9