Hotspot

rrn4.5-rrn5

IRb · 108506-108732 · 227 bp

Back to Aponogeton

Pi 0.0499

MarkerSeek score 71.80

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 227
variable_sites: 17
pi: 0.0499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	344 bp	1.0	83.3
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	342 bp	1.0	83.3
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	340 bp	1.0	83.3
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	368 bp	1.0	83.1
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	370 bp	1.0	83.0