Hotspot

psaJ-rpl33

LSC · 68489-68941 · 453 bp

Back to Aponogeton

Pi 0.0316

MarkerSeek score 75.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 453
variable_sites: 21
pi: 0.0316
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACGCCTTGTCATTTCCCTTT	CCGGCGATTCCTTATTGACA	45.0	50.0	58.0	58.1	551-564 bp	1.0	85.1
psaJ-rpl33_p2	ATCGTTTATTCCCAGACGCC	CCGGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	566-579 bp	1.0	84.6
psaJ-rpl33_p3	ACGCCTTGTCATTTCCCTTT	CCTTTCGAGCACAACAGGTA	45.0	50.0	58.0	57.8	530-543 bp	1.0	84.4
psaJ-rpl33_p4	ATCGTTTATTCCCAGACGCC	CCTTTCGAGCACAACAGGTA	50.0	50.0	58.1	57.8	545-558 bp	1.0	83.9
psaJ-rpl33_p5	ATCTCTCTGTGGCACCTGTA	CCGGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	635-648 bp	1.0	82.5