Hotspot

accD-psaI

LSC · 59872-60717 · 846 bp

Back to Aponogeton

Pi 0.0221

MarkerSeek score 69.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 28
pi: 0.0221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1001-1014 bp	1.0	83.7
accD-psaI_p2	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	1007-1020 bp	1.0	83.0
accD-psaI_p3	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	949-962 bp	1.0	82.9
accD-psaI_p4	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	996-1009 bp	1.0	82.7
accD-psaI_p5	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	995-1008 bp	1.0	82.7