Hotspot

rps4-trnT-UGU

LSC · 46691-47082 · 392 bp

Back to Aponogeton

Pi 0.0376

MarkerSeek score 71.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 22
pi: 0.0376
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	55.4	57.9	498-509 bp	1.0	47.9
rps4-trnT-UGU_p2	CCCAGACGGCGTATTTTT	GGTTCGATTCCGATAGCTGG	50.0	55.0	55.4	58.2	457-468 bp	1.0	47.8
rps4-trnT-UGU_p3	CCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	55.4	57.8	466-477 bp	1.0	47.5
rps4-trnT-UGU_p4	CCCAGACGGCGTATTTTT	CTTTTACGTGAGCCCGCTTA	50.0	50.0	55.4	58.3	519-530 bp	1.0	46.5
rps4-trnT-UGU_p5	CCCAGACGGCGTATTTTT	TTTTACGTGAGCCCGCTTAG	50.0	50.0	55.4	58.3	518-529 bp	1.0	46.5