Hotspot

ycf3-trnS-GGA

LSC · 45358-45703 · 346 bp

Back to Aponogeton

Pi 0.0344
MarkerSeek score 71.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
346
variable_sites
17
pi
0.0344
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGGCTATAATTGAGGAGGTCT TTCCAATGCTACGCCTTGAA 42.9 45.0 55.5 57.8 433-442 bp 1.0 57.9
ycf3-trnS-GGA_p2 TGGCTATAATTGAGGAGGTCT ACGGAAAGAGAGGGATTCGA 42.9 50.0 55.5 58.2 484-493 bp 1.0 57.5
ycf3-trnS-GGA_p3 TGGCTATAATTGAGGAGGTCTT TTCCAATGCTACGCCTTGAA 40.9 45.0 56.3 57.8 433-442 bp 1.0 56.1
ycf3-trnS-GGA_p4 TGGCTATAATTGAGGAGGTCTT ACGGAAAGAGAGGGATTCGA 40.9 50.0 56.3 58.2 484-493 bp 1.0 55.7
ycf3-trnS-GGA_p5 TTGGCTATAATTGAGGAGGTCT ACGGAAAGAGAGGGATTCGA 40.9 50.0 56.3 58.2 485-494 bp 1.0 55.7